TIFF is a very flexible file format. Therefore, you have several different settings.
Format for Saving allows to choose the compression for saving. You have the choice between TIF unpacked, TIF LZW-packed, TIF JPEG-packed, TIF Packbits, TIF Class 3, TIF Class 4 and TIF ZIP.
The slider JPEG Compression controls the reduction of the JPEG compressor. A value of 100 means minimum compression and highest image quality, a value of 0 means maximum compression and lowest quality. If you save an image for displaying, on the screen a value of 70 is appropriate, for printing you should use 90. But pay attention: JPEG is lossy and only few applications are able to read TIF files that are compressed with JPEG.
Using Save Layer/Page Images the saving of pictures consisting of several layers is controlled. If Normal Image is activated, the picture will be reduced to one layer and this one will be saved. With Save Layers each layer will be saved as a separate image in the TIFF-file. But note, that many applications interpret TIFF-files with several images as multi-page documents. If you turn on Ask, on saving you will be asked whether to reduce the picture to one layer.
There are TIFF-files, that contain several images. If you select Open Multipage TIFFs on Pages, PhotoLine will create a single page for every image in the file. Otherwise you will get one page with several layers.